LOCUS       pLV-[hsa-miR-1231]        9146 bp    DNA     circular      2-MAR-2011
SOURCE      
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7067..7927
                     /label=Amp(R)
     promoter        8128..8668
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5672..5906
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6481..6936)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5978..6108
                     /label=SV40 pA
     misc_feature    6109..6322
                     /label=SV40 ori
     misc_feature    4993..5589
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3611
                     /label=intron
     CDS             3658..4983
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3401
                     /label=hsa-miR-1231 precursor
BASE COUNT     2166 a      2390 c      2489 g      2101 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg ctatgggaga 
     3121 aggaaatgaa gcttacagac atccgcttgg aggccctcaa ctctgcccac caactggatc 
     3181 agcttcggga gaccatgcac aacatgcagg tcagtgtctg ggcggacagc tgcaggaaag 
     3241 ggaagaccaa ggcttgctgt ctgtccagtc tgccacccta ccctgtctgt tcttgccaca 
     3301 gttggaggtg gacctgctga aagcagagaa tgaccgactg aaggtagccc caggcccctc 
     3361 atcaggctcc actccagggc aggtccctgg atcatctgca tgctagctcg agcttttgga 
     3421 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 
     3481 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 
     3541 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 
     3601 ttccatttca ggtgtcgtga aaactacccc tctagagtcg agctaccggt cgccaccatg 
     3661 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg 
     3721 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 
     3781 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc 
     3841 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc 
     3901 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 
     3961 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc 
     4021 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg 
     4081 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc 
     4141 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag 
     4201 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac 
     4261 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc 
     4321 gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggaccc accggtcgcc 
     4381 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 
     4441 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 
     4501 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 
     4561 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 
     4621 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 
     4681 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 
     4741 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 
     4801 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 
     4861 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 
     4921 gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc 
     4981 tgagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 
     5041 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 
     5101 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 
     5161 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 
     5221 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 
     5281 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 
     5341 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 
     5401 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 
     5461 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 
     5521 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 
     5581 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga 
     5641 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 
     5701 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 
     5761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 
     5821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 
     5881 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 
     5941 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 
     6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 
     6121 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     6181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     6241 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata 
     6301 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 
     6361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 
     6421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg 
     6481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     6541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     6601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     6661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     6721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     6781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     6841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     6901 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     7021 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     7081 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     7141 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     7201 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     7261 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 
     7321 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     7381 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     7441 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     7501 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     7561 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 
     7621 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     7681 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     7741 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     7801 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     7861 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     7921 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     7981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     8461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     8521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     8581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     8641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     8701 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     8761 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 
     8821 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 
     8881 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 
     8941 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 
     9001 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 
     9061 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca 
     9121 ctaaagggaa caaaagctgg agctgc 
//
